Incubation:Article Title: Anti-CD166 antibodies, activatable anti-CD166 antibodies, and methods of use thereof
Article Snippet: .. T1234201, T1234086, T1234183, T1234188, and T1234127, respectively; BioChain, Newark, Calif.) were prepared and blocked using standard protocols, and then incubated with 0.4 μg/mL with an anti-CD166 ADC of the present disclosure (anti-CD166-spdb-DM4), an anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4), an activated anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4 incubated with purified uPA for 16 hours at 37° C.), or an isotype control ADC (chKTI-spdb-DM4, a chimeric human IgG1 anti-soybean trypsin inhibitor antibody, conjugated to spdb-DM4). .. After incubation with the test articles, sections were treated with 2.5 μg/mL mouse anti-DM4 monoclonal antibody (Immunogen).
Article Title: Anti-CD166 antibodies, activatable anti-CD166 antibodies, and methods of use thereof
Article Snippet: .. T1234201, T1234086, T1234183, T1234188, and T1234127, respectively; BioChain, Newark, Calif.) were prepared and blocked using standard protocols, and then incubated with 0.4 μg/mL with an anti-CD166 ADC of the present disclosure (anti-CD166-spdb-DM4), an anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4), an activated anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4 incubated with purified uPA for 16 hours at 37° C.), or an isotype control ADC (chKTI-spdb-DM4, a chimeric human IgG1 anti-soybean trypsin inhibitor antibody, conjugated to spdb-DM4). .. After incubation with the test articles, sections were treated with 2.5 μg/mL mouse anti-DM4 monoclonal antibody (Immunogen).
Purification:Article Title: Anti-CD166 antibodies, activatable anti-CD166 antibodies, and methods of use thereof
Article Snippet: .. T1234201, T1234086, T1234183, T1234188, and T1234127, respectively; BioChain, Newark, Calif.) were prepared and blocked using standard protocols, and then incubated with 0.4 μg/mL with an anti-CD166 ADC of the present disclosure (anti-CD166-spdb-DM4), an anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4), an activated anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4 incubated with purified uPA for 16 hours at 37° C.), or an isotype control ADC (chKTI-spdb-DM4, a chimeric human IgG1 anti-soybean trypsin inhibitor antibody, conjugated to spdb-DM4). .. After incubation with the test articles, sections were treated with 2.5 μg/mL mouse anti-DM4 monoclonal antibody (Immunogen).
Article Title: Anti-CD166 antibodies, activatable anti-CD166 antibodies, and methods of use thereof
Article Snippet: .. T1234201, T1234086, T1234183, T1234188, and T1234127, respectively; BioChain, Newark, Calif.) were prepared and blocked using standard protocols, and then incubated with 0.4 μg/mL with an anti-CD166 ADC of the present disclosure (anti-CD166-spdb-DM4), an anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4), an activated anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4 incubated with purified uPA for 16 hours at 37° C.), or an isotype control ADC (chKTI-spdb-DM4, a chimeric human IgG1 anti-soybean trypsin inhibitor antibody, conjugated to spdb-DM4). .. After incubation with the test articles, sections were treated with 2.5 μg/mL mouse anti-DM4 monoclonal antibody (Immunogen).
Control:Article Title: Anti-CD166 antibodies, activatable anti-CD166 antibodies, and methods of use thereof
Article Snippet: .. T1234201, T1234086, T1234183, T1234188, and T1234127, respectively; BioChain, Newark, Calif.) were prepared and blocked using standard protocols, and then incubated with 0.4 μg/mL with an anti-CD166 ADC of the present disclosure (anti-CD166-spdb-DM4), an anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4), an activated anti-CD166 AADC of the present disclosure (anti-CD166-7614.6-3001-spdb-DM4 incubated with purified uPA for 16 hours at 37° C.), or an isotype control ADC (chKTI-spdb-DM4, a chimeric human IgG1 anti-soybean trypsin inhibitor antibody, conjugated to spdb-DM4). .. After incubation with the test articles, sections were treated with 2.5 μg/mL mouse anti-DM4 monoclonal antibody (Immunogen).
|
